活性酶普遍存在于各种生命活动中，一些疾病与活性酶的异常表达息息相关，精确检测酶的表达水平以及原位成像，为相关疾病的诊断与治疗提供了有力的判断依据。至今，大量的检测技术已经开发出来，其中以分子荧光探针为代表的光学技术具有非侵袭性以及灵敏度高、检测限低、响应时间快和生物相容性好等优势，在检测活性酶上备受青睐。然而，在使用分子荧光探针检测时，由于小分子容易在酶活性位点发生扩散，无法定位，导致探针时空分辨率较差。因此，为提高成像检测的时空分辨率、降低背景干扰和假阳性，原位成像的设计理念随之提出，已成为生物光学成像的研究热点之一。目前，研究者已报道多种分子荧光探针用于酶的原位成像的设计并取得显著效果。本文将深入介绍用于活性酶检测的分子荧光探针的设计策略及其在原位成像中的研究进展，希望为该领域的研究者们提供一些启发。

Dealing with the increase in data workloads and network complexity requires efficient selective manipulation of any channels in hybrid mode-/wavelength-division multiplexing (MDM/WDM) systems. A reconfigurable optical add-drop multiplexer (ROADM) using special modal field redistribution is proposed and demonstrated to enable the selective access of any mode-/wavelength-channels. With the assistance of the subwavelength grating structures, the launched modes are redistributed to be the supermodes localized at different regions of the multimode bus waveguide. Microring resonators are placed at the corresponding side of the bus waveguide to have specific evanescent coupling of the redistributed supermodes, so that any mode-/wavelength-channel can be added/dropped by thermally tuning the resonant wavelength. As an example, a ROADM for the case with three mode-channels is designed with low excess losses of <0.6, 0.7, and 1.3 dB as well as low cross talks of < - 26.3, -28.5, and -39.3 dB for the TE₀, TE₁, and TE₂ modes, respectively, around the central wavelength of 1550 nm. The data transmission of 30 Gbps / channel is also demonstrated successfully. The present ROADM provides a promising route for data switching/routing in hybrid MDM/WDM systems.

The collective response of macroscopic quantum states under perturbation is widely used to study quantum correlations and cooperative properties, such as defect-induced quantum vortices in Bose–Einstein condensates and the non-destructive scattering of impurities in superfluids. Superfluorescence (SF), as a collective effect rooted in dipole–dipole cooperation through virtual photon exchange, leads to the macroscopic dipole moment (MDM) in high-density dipole ensembles. However, the perturbation response of the MDM in SF systems remains unknown. Echo-like behavior is observed in a cooperative exciton ensemble under a controllable perturbation, corresponding to an initial collapse followed by a revival of the MDM. Such a dynamic response could refer to a phase transition between the macroscopic coherence regime and the incoherent classical state on a time scale of 10 ps. The echo-like behavior is absent above 100 K due to the instability of MDM in a strongly dephased exciton ensemble. Experimentally, the MDM response to perturbations is shown to be controlled by the amplitude and injection time of the perturbations.

Optical physical unclonable functions (PUFs) have emerged as a promising strategy for effective and unbreakable anti-counterfeiting. However, the unpredictable spatial distribution and broadband spectra of most optical PUFs complicate efficient and accurate verification in practical anti-counterfeiting applications. Here, we propose an optical PUF-based anti-counterfeiting label from perovskite microlaser arrays, where randomness is introduced through vapor-induced microcavity deformation. The initial perovskite microdisk laser arrays with regular positions and uniform sizes are fabricated by femtosecond laser direct ablation. By introducing vapor fumigation to induce random deformations in each microlaser cavity, a laser array with completely uneven excitation thresholds and narrow-linewidth lasing signals is obtained. As a proof of concept, we demonstrated that the post-treated laser array can provide fixed-point and random lasing signals to facilitate information encoding. Furthermore, different emission states of the lasing signal can be achieved by altering the pump energy density to reflect higher capacity information. A threefold PUF (excited under three pump power densities) with a resolution of 5×5 pixels exhibits a high encoding capacity (1.43×1045), making it a promising candidate to achieve efficient authentication and high security with anti-counterfeiting labels.

Lead halide perovskite microlasers have shown impressive performance in the green and red wavebands. However, there has been limited progress in achieving blue-emitting perovskite microlasers. Here, blue-emitting perovskite-phase rubidium lead bromide (RbPbBr3) microcubes were successfully prepared by using a one-step chemical vapor deposition process, which can be utilized to construct optically pumped whispering gallery mode microlasers. By regulating the growth temperature, we found that a high-temperature environment can facilitate the formation of the perovskite phase and microcubic morphology of RbPbBr3. Notably, blue single-mode lasing in a RbPbBr3 microcubic cavity with a narrow linewidth of 0.21 nm and a high-quality factor (∼2200) was achieved. The obtained lasing from RbPbBr3 microlasers also exhibited an excellent polarization state factor (∼0.77). By modulating the mixed-monovalent cation composition, the wavelength of the microlaser could be tuned from green (536 nm) to pure blue (468 nm). Additionally, the heat stability of the mix-cation perovskite was better than that of conventional CsPbBr3. The stable and high-performance blue single-mode microlasers may thus facilitate the application of perovskite lasers in blue laser fields.

Chip-scale programmable optical signal processors are often used to flexibly manipulate the optical signals for satisfying the demands in various applications, such as lidar, radar, and artificial intelligence. Silicon photonics has unique advantages of ultra-high integration density as well as CMOS compatibility, and thus makes it possible to develop large-scale programmable optical signal processors. The challenge is the high silicon waveguides propagation losses and the high calibration complexity for all tuning elements due to the random phase errors. In this paper, we propose and demonstrate a programmable silicon photonic processor for the first time by introducing low-loss multimode photonic waveguide spirals and low-random-phase-error Mach-Zehnder switches. The present chip-scale programmable silicon photonic processor comprises a 1×4 variable power splitter based on cascaded Mach-Zehnder couplers (MZCs), four Ge/Si photodetectors, four channels of thermally-tunable optical delaylines. Each channel consists of a continuously-tuning phase shifter based on a waveguide spiral with a micro-heater and a digitally-tuning delayline realized with cascaded waveguide-spiral delaylines and MZSs for 5.68 ps time-delay step. Particularly, these waveguide spirals used here are designed to be as wide as 2 μm, enabling an ultralow propagation loss of 0.28 dB/cm. Meanwhile, these MZCs and MZSs are designed with 2-μm-wide arm waveguides, and thus the random phase errors in the MZC/MZS arms are negligible, in which case the calibration for these MZSs/MZCs becomes easy and furthermore the power consumption for compensating the phase errors can be reduced greatly. Finally, this programmable silicon photonic processor is demonstrated successfully to verify a number of distinctively different functionalities, including tunable time-delay, microwave photonic beamforming, arbitrary optical signal filtering, and arbitrary waveform generation.

A compact on-chip reconfigurable multichannel amplitude equalizer based on cascaded elliptical microrings is proposed and demonstrated experimentally. With the optimized structure of the elliptical microring with adiabatically varied radii/widths, the average excess loss for each channel in the initialized state is measured to be less than 0.5 dB, while the attenuation dynamic range can be over 20 dB. Flexible tunability through the overlapping of the resonance peaks of adjacent wavelength-channels enables even higher attenuation dynamic ranges up to 50 dB. Leveraging the thermo-optic effect and fine wavelength-tuning linearity, precise tuning of the resonance peak can be implemented, enabling dynamic power equalization of each wavelength-channel in wavelength-division-multiplexing (WDM) systems and optical frequency combs. The proposed architecture exhibits excellent scalability, which can facilitate the development of long-haul optical transport networks and high-capacity neuromorphic computing systems, while improving the overall performance of optical signals in WDM-related systems.